Download e-book for kindle: Signal Transduction Protocols by Louis M. Luttrell, Stephen S. G. Ferguson

By Louis M. Luttrell, Stephen S. G. Ferguson

ISBN-10: 1617791598

ISBN-13: 9781617791598

ISBN-10: 1617791601

ISBN-13: 9781617791604

It is more and more transparent that sign transduction is a hugely geared up and built-in approach. Divided into handy sections, Signal Transduction Protocols, 3rd Edition specializes in experimental methods to raised comprehend the complexity of sign transduction. Introductory chapters offer viewpoint on a number of of the demanding situations fascinated about sign transduction examine and provide information on selecting the right methods to varied sorts of questions. the person chapters supply unique experimental protocols masking numerous subject matters, from the consequences of ligand binding on receptor conformation and effector coupling, to relocating contained in the telephone with a view to seize the spatial and temporal features of signaling occasions. Written within the hugely winning Methods in Molecular Biology™ sequence structure, chapters include introductions to their respective subject matters, lists of the required fabrics and reagents, step-by step, with no trouble reproducible laboratory protocols, and notes on troubleshooting and fending off recognized pitfalls.

Authoritative and accessible, sign Transduction Protocols serves as a fantastic advisor to scientists of all backgrounds and is a helpful source for destiny development within the box of sign transduction research.

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Prot Sci 9, 10–9. 81. Hilser, V. , Garcia-Moreno, E. , Oas, T. , and Whitten, S. T. (2006) A statistical thermodynamic model of protein ensembles. Chem Rev 106, 1545–58. 82. Livesay, D. , Wood, G. , and Jacobs, D. J. (2004) A flexible approach for understanding protein stability. FEBS Lett. 576, 468–76. 83. Hilser, V. , and Thompson, E. B. (2007) Intrinsic disorder as a mechanism to optimize allosteric coupling in proteins. Proc Natl Acad Sci USA 104, 8311–5. 84. Kobilka, B. , and Deupi, X. (2007) Conformational complexity of G-proteincoupled receptors.

And Jakobs, K. H. (1994) Transfected muscarinic acetylcholine receptors selectively couple to Gi-type G proteins and Gq/11. Mol Pharmacol 45, 890–898. 34. Kenakin, T. (1995) Agonist-receptor efficacy. II. Agonist-trafficking of receptor signals. Trends Pharmacol Sci 16, 232–38. 35. , Lieu, H. , and Bohmaker, K. (1992) Comparative effects of receptor inactivation, 17 b-estradiol and pertussis toxin on dopaminergic inhibition of prolactin secretion in vitro. J Pharmacol Exp Ther 263, 462–9. 36.

If the objective is an unbiased screen for whether compounds exert any form of activity against a receptor, then technology designed to detect integrated whole cell pharmacologic responses may provide the first step. Resonant waveguide grating technology has led to the development of optical biosensors that can measure dynamic mass redistribution (DMR) signals in living cells (140, 141). This technology can detect interactions of GPCRs with cytosolic signaling molecules at a depth of 150–200 nM and also detect receptor internalization.

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Signal Transduction Protocols by Louis M. Luttrell, Stephen S. G. Ferguson

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