According to examine and effects from the foreign Human Kidney and Urine Proteome undertaking, this guide and reference offers present tools and protocols for the research of human kidney and urine tract. The world's prime specialists hide every thing from uncomplicated innovations for regimen research to complicated equipment for tough samples or analytes. Written with the practitioner in brain, the entire equipment and strategies are defined with step by step protocols subsidized through important functional suggestion taken from genuine existence experiments.
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Extra resources for Renal and Urinary Proteomics: Methods and Protocols
The material that emerged through the sieve with cold saline was collected in a receiving pan on ice. The sieved material was gently shaken, and then poured on top of a #80 (184 µm) mesh sieve that was positioned on top of a #150 (103 µm) mesh sieve, rested on a receiving pan. These sieves were washed in the same sequence with ice-cold saline and the glomeruli retained on the #150 mesh sieve were free of tubular fragments and cells. The glomeruli were then suspended in ice-cold saline or PBS, transferred to a 50-ml centrifuge tube, and left standing on ice for 10–15 minutes.
And Burg, M. (1986) Predominant osmotically active organic solutes in rat and rabbit renal medullas. J Biol Chem, 261, 5872–5877. , and Muller, E. (2000) Molecular chaperones in the kidney: distribution, putative roles, and regulation. Am J Physiol Renal Physiol, 279, F203–F215. A. (2005) Proteomic analysis of cellular response to osmotic stress in thick ascending limb of Henle’s loop (TALH) cells. Mol Cell Proteomics, 4, 1445–1458. B. (1995) Molecular basis of osmotic regulation. Am J Physiol, 268, F983–F996.
1976) A new method for the isolation of ultrastructurally preserved glomeruli. Kidney Int, 9, 278–285. , and Yamamoto, T. (2001) Phenotypic modulation of parietal epithelial cells of Bowman’s capsule in culture. Cell Tissue Res, 304, 339–349. 7 19 3 Isolation and Analysis of Thick Ascending Limb of Henle’s Loop (TALH) Cells Hassan Dihazi and Gerhard A. 1 Introduction The kidney is a heterogeneous organ. The functional unit is the nephron, which is composed of a multitude of cell types with widely differing structures and functions.
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