Renal and Urinary Proteomics: Methods and Protocols by Thongboonkerd PDF

By Thongboonkerd

ISBN-10: 3527319743

ISBN-13: 9783527319749

According to examine and effects from the foreign Human Kidney and Urine Proteome undertaking, this guide and reference offers present tools and protocols for the research of human kidney and urine tract. The world's prime specialists hide every thing from uncomplicated innovations for regimen research to complicated equipment for tough samples or analytes. Written with the practitioner in brain, the entire equipment and strategies are defined with step by step protocols subsidized through important functional suggestion taken from genuine existence experiments.

Show description

Read or Download Renal and Urinary Proteomics: Methods and Protocols PDF

Similar nonfiction_5 books

Download e-book for kindle: Stesichoros's Geryoneis (Mnemosyne Supplements - Volume 333) by Paul Curtis

Stesichoross Geryoneis is likely one of the gemstones of the sixth century. This monograph bargains the 1st full-length remark (in English) to hide all features of the Geryoneis. integrated during this monograph is a much-needed revised and updated textual content including an entire equipment. in addition to targeting the poets utilization of metre and language, a specific emphasis has been given to Stesichoross debt to epic poetry.

New PDF release: Behavioral Sport Psychology: Evidence-Based Approaches to

Behavioral game PsychologyEvidence-Based methods to functionality EnhancementJames okay. Luiselli and Derek D. Reed, editorsFrom its fringe beginnings within the Nineteen Sixties, activity psychology has developed right into a mainstream forte, encompassing motivation, self belief construction, blunders relief, and self-help instruments, between others.

Extra resources for Renal and Urinary Proteomics: Methods and Protocols

Example text

The material that emerged through the sieve with cold saline was collected in a receiving pan on ice. The sieved material was gently shaken, and then poured on top of a #80 (184 µm) mesh sieve that was positioned on top of a #150 (103 µm) mesh sieve, rested on a receiving pan. These sieves were washed in the same sequence with ice-cold saline and the glomeruli retained on the #150 mesh sieve were free of tubular fragments and cells. The glomeruli were then suspended in ice-cold saline or PBS, transferred to a 50-ml centrifuge tube, and left standing on ice for 10–15 minutes.

And Burg, M. (1986) Predominant osmotically active organic solutes in rat and rabbit renal medullas. J Biol Chem, 261, 5872–5877. , and Muller, E. (2000) Molecular chaperones in the kidney: distribution, putative roles, and regulation. Am J Physiol Renal Physiol, 279, F203–F215. A. (2005) Proteomic analysis of cellular response to osmotic stress in thick ascending limb of Henle’s loop (TALH) cells. Mol Cell Proteomics, 4, 1445–1458. B. (1995) Molecular basis of osmotic regulation. Am J Physiol, 268, F983–F996.

1976) A new method for the isolation of ultrastructurally preserved glomeruli. Kidney Int, 9, 278–285. , and Yamamoto, T. (2001) Phenotypic modulation of parietal epithelial cells of Bowman’s capsule in culture. Cell Tissue Res, 304, 339–349. 7 19 3 Isolation and Analysis of Thick Ascending Limb of Henle’s Loop (TALH) Cells Hassan Dihazi and Gerhard A. 1 Introduction The kidney is a heterogeneous organ. The functional unit is the nephron, which is composed of a multitude of cell types with widely differing structures and functions.

Download PDF sample

Renal and Urinary Proteomics: Methods and Protocols by Thongboonkerd

by Daniel

Rated 4.73 of 5 – based on 25 votes