By Michael J. Soares, Joan S. Hunt
Placenta learn has advanced quickly during the last a number of a long time via profiting from technical advances, reminiscent of microarray research, opposite transcriptase polymerase chain response, protein research, and in situ hybridization. In Placenta and Trophoblast: tools and Protocols, Volumes 1 & 2, across the world famous investigators describe state-of-the-art laboratory ideas for the examine of the trophoblast and placental biology. The concepts provided diversity from experimental animal types, to animal and human placental organ and cellphone tradition structures, to morphological, biochemical, and molecular thoughts for assessing trophoblast/placental progress, differentiation, and serve as. quantity 1 offers with ease reproducible protocols for learning embryo-uterine implantation, trophoblast cellphone improvement, and the association and molecular characterization of the placenta. Highlights comprise techniques for the isolation and tradition of trophoblast cells from primates, ruminants, and rodents, and special counsel to the molecular and mobile research of the placental phenotype. A spouse moment quantity concentrates on equipment for investigating placental functionality.
finished and state of the art, Placenta and Trophoblast: tools and Protocols, Volumes 1 & 2 offer researchers an organization starting place for profitable mobile and molecular research of the placenta and the institution of pregnancy.
Read or Download Placenta and Trophoblast: Methods and Protocols Volume 1 PDF
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Additional resources for Placenta and Trophoblast: Methods and Protocols Volume 1
1. Subcutaneous Injection (Anesthesia Not Required) 1. Grasp the base of the tail with the thumb and forefinger of one hand. 2. Place the mouse on the top of the cage cover (wire top). Clear the back of the neck with an alcohol swab. Mouse Implantation Methodologies 31 3. Hold the syringe attached to a 27-gauge needle parallel to the head. 4. As the mouse attempts to move forward, quickly insert the tip of the needle in the scruff (loose skin on the back of the neck) at a very shallow angle and lift the skin with the needle to avoid underlying muscle.
Check the diameter of the pulled pipet under a microscope and break the end at a place where the diameter will be greater (approx 200 μm) than the size of a blastocyst. One can use an oilstone to mark the breaking point of the glass for an even tip. 3. Fire-polish the tip of the pipet by quickly touching the flame (an uneven tip may damage the blastocyst and the uterus). 4. Bend the tube (at a 120–130° angle) over a flame about one-half of an inch from the unpulled end. 5. Place the unpulled end of the pipet inside a 16-gauge steel needle and seal it (make it air-tight) with “super” glue.
8. 9. 10. 11. 12. 13. 14. 15. 16. Day 4 pseudopregnant or progesterone-treated ovariectomized mice. Anesthesia (Avertin). Paper towels. Animal clipper (Fisher Scientific, cat. no. 01-305-10). ). ). , cat. nos. 11153-10 and 11150-10). , cat. nos. 14558-11). 23-gauge BD PrecisionGlide needle (Fisher Scientific, cat. no. 14-826A). BD Autoclip wound clips (Fisher Scientific, cat. no. 01-804-5) and applier (Fisher Scientific, cat. no. 01-804). , Model No. 26020). 9% sodium chloride, Baxter Healthcare Corporation, cat.
Placenta and Trophoblast: Methods and Protocols Volume 1 by Michael J. Soares, Joan S. Hunt