By Melissa Yeung MD, FRCPC (auth.), Anil Chandraker, Mohamed H. Sayegh, Ajay K. Singh (eds.)
Though kidney transplantation is taken into account a regimen method, there are nonetheless major demanding situations in post-transplant administration. Core ideas in Renal Transplantation is a clinically centred authoritative consultant to the administration of kidney transplantation. This finished, cutting-edge reference summarizes the new adjustments within the box of transplantation, providing the entire variety of updated details on the entire a variety of points of easy immunobiology and the treatment of the transplant recipient. Written through a crew of popular gurus in renal transplantation, this concise source is meant for either the nephrologist and the non-specialist with an curiosity in kidney transplantation.
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Identical) and yet the recipient could form an antibody to the epitope with the amino acid difference that would not be expected based upon serologic typing alone. Molecular Typing HLA proteins are encoded by DNA regions on the short arm of chromosome 6. With their sequences well described  molecular typing methods are increasingly used including sequence specific primer polymerase chain reaction (SSP-PCR), sequence specific oligonucleotide probes (SSOP), and direct DNA sequencing. In SSP-PCR, DNA is isolated from the subject to be typed, and amplified in multiple wells, each containing specific primers complementary to particular HLA alleles.
The desire to discriminate HLA antibody from non-HLA antibody, as well as to clearly differentiate Class I and Class II antibodies stimulated the development of the currently available solid phase methodologies. These methods utilize only soluble or recombinant HLA molecules rather than lymphocyte targets which present both HLA and non-HLA molecules. Purified HLA molecules are applied to solid phase media (enzyme-linked immunosorbent assay [ELISA] [20, 21] platforms or microbeads ), and therefore will bind only HLA antibody when recipient serum is added.
J. Tinckam 3. von Andrian UH, Mempel TR. Homing and cellular traffic in lymph nodes. Nat Rev Immunol. 2003;3(11):867–78. 4. Ono SJ, et al. Chemokines: roles in leukocyte development, trafficking, and effector function. J Allergy Clin Immunol. 2003;111(6):1185–99; quiz 1200. 5. Cyster JG. Homing of antibody secreting cells. Immunol Rev. 2003;194:48–60. 6. Jacobelli J et al. New views of the immunological synapse: variations in assembly and function. Curr Opin Immunol. 2004;16(3):345–52. 7. von Andrian UH, Mackay CR.
Core Concepts in Renal Transplantation by Melissa Yeung MD, FRCPC (auth.), Anil Chandraker, Mohamed H. Sayegh, Ajay K. Singh (eds.)