By Arun Kumar Sharma
Read Online or Download Chromosome Techniques. Theory and Practice PDF
Best medical books books
Half One THE CONQUEST OF demise AT delivery I. Childbirth and Civilization 3T he care given the child-bearing girl; an index of civilization Medieval indifference to the affliction of girls Baptism ahead of delivery Filthy a while of religion stipulations for the lady of this day Leaders within the conquest of dying The cultural lag Low place of girls within the usa How primitive ladies undergo their childrens Why they've got few problems The harmful impacts of civilization -N ative confinements Customs of purification exertions believed a voluntary act at the childs half tough information in tricky laborS aliva as a therapeutic agent the increase of the midwives Early rules to regulate midwives The obstetrical chair Innovation of utilizing a mattress for childbirth Indian obstetrical practices the medication of the traditional Greeks Legends of y!
Medication tells the attention-grabbing tale of the self-discipline, from precedent days to the current day, charting advancements in therapeutic, analysis, surgical procedure, and medicine in a vividly visible and available structure. keep on with the gory pitfalls and the staggering breakthroughs of clinical heritage from trepanning, bloodletting, and physique snatching to the most recent advancements in IVF and gene treatment.
Additional info for Chromosome Techniques. Theory and Practice
Estac: esp. Aula Dei 2, 21 Tsunewaki, K. and Jenkins, B. C. (1960). Cytologia 25, 373 Windaus, A. (1924). Liebigs Ann. 439, 59 Wolff, S. and Luippold, H. E. (1956). Stain Tech. 3 1 , 201 Zeisel, S. (1883). Mh. Chem. 4, 162 3 Fixation Fixation may be defined as the process by which tissues or their components are fixed selectively at a particular stage to a desired extent. The purpose of fixation is to kill the tissue without causing any distortion of the components to be studied, as far as is practicable.
Methanol (CH OH) 3 It is occasionally used in chromosome studies of plants but is extensively employed in fixing animal chromosomes. It is obtained from destructive distillation of wood, as a component of pyroligneous acid, but does not possess the essential requirements of a typical alcoholic fixative. While ethanol causes heavy shrinkage of chromosomes, methanol causes swelling and this property has been used advantageously in the preparation of fixatives where a swelling agent is often needed to compensate for the shrinking effect of other chemicals.
The solvent ethanol must be removed for pre-treatment schedules. It is preferable to use solutions in water. The main effects of this chemical are: immediate cessation of mitotic activity, followed by contraction of chromosomes in prophase, metaphase and anaphase. In some cases, the chromosomes may be reduced to about one quarter of their original length (Mann, Jordan and Day, 1965; Storey and M a n n , 1967). Sawamura (1965) noted that CIPC, a m-chloroanalogue of IPC, causes inhibition of phragmoplast and spindle development.
Chromosome Techniques. Theory and Practice by Arun Kumar Sharma