Chromosome Techniques. Theory and Practice by Arun Kumar Sharma PDF

By Arun Kumar Sharma

ISBN-10: 0408709421

ISBN-13: 9780408709422

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Estac: esp. Aula Dei 2, 21 Tsunewaki, K. and Jenkins, B. C. (1960). Cytologia 25, 373 Windaus, A. (1924). Liebigs Ann. 439, 59 Wolff, S. and Luippold, H. E. (1956). Stain Tech. 3 1 , 201 Zeisel, S. (1883). Mh. Chem. 4, 162 3 Fixation Fixation may be defined as the process by which tissues or their components are fixed selectively at a particular stage to a desired extent. The purpose of fixation is to kill the tissue without causing any distortion of the components to be studied, as far as is practicable.

Methanol (CH OH) 3 It is occasionally used in chromosome studies of plants but is extensively employed in fixing animal chromosomes. It is obtained from destructive distillation of wood, as a component of pyroligneous acid, but does not possess the essential requirements of a typical alcoholic fixative. While ethanol causes heavy shrinkage of chromosomes, methanol causes swelling and this property has been used advantageously in the preparation of fixatives where a swelling agent is often needed to compensate for the shrinking effect of other chemicals.

The solvent ethanol must be removed for pre-treatment schedules. It is preferable to use solutions in water. The main effects of this chemical are: immediate cessation of mitotic activity, followed by contraction of chromosomes in prophase, metaphase and anaphase. In some cases, the chromosomes may be reduced to about one quarter of their original length (Mann, Jordan and Day, 1965; Storey and M a n n , 1967). Sawamura (1965) noted that CIPC, a m-chloroanalogue of IPC, causes inhibition of phragmoplast and spindle development.

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Chromosome Techniques. Theory and Practice by Arun Kumar Sharma


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